Fig. 2. Pharmacological inhibition of acid sphingomyelinase prevents EAE. SJL/J and C57BL/6 mice were immunized for experimental autoimmune encephalomyelitis (EAE) and treated with amitriptyline starting on day 1 after immunization. The disease was scored daily. Shown is a clinical course of one representative EAE experiment of each mouse strain (a) and the incidence of active EAE clinical symptom development (b). Shown are means ± SD, n = 5 for C57BL/6 and n= 11 for SJL/J mice.